SnRK1 from Arabidopsis thaliana is an atypical AMPK

S Emanuelle; MI Hossain; IE Moller; HL Pedersen; AML Van De Meene; MS Doblin; A Koay; JS Oakhill; JW Scott; WGT Willats; BE Kemp; A Bacic; PR Gooley; DI Stapleton

Journal article

SnRK1 from Arabidopsis thaliana is an atypical AMPK

S Emanuelle, MI Hossain, IE Moller, HL Pedersen, AML Van De Meene, MS Doblin, A Koay, JS Oakhill, JW Scott, WGT Willats, BE Kemp, A Bacic, PR Gooley, DI Stapleton

Plant Journal | Published : 2015

DOI: 10.1111/tpj.12813

Abstract

Summary SNF1-related protein kinase 1 (SnRK1) is the plant orthologue of the evolutionarily-conserved SNF1/AMPK/SnRK1 protein kinase family that contributes to cellular energy homeostasis. Functional as heterotrimers, family members comprise a catalytic α subunit and non-catalytic β and γ subunits; multiple isoforms of each subunit type exist, giving rise to various isoenzymes. The Arabidopsis thaliana genome contains homologues of each subunit type, and, in addition, two atypical subunits, β3 and βγ, with unique domain architecture, that are found only amongst plants, suggesting atypical heterotrimers. The AtSnRK1 subunit structure was determined using recombinant protein expression and end..

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University of Melbourne Researchers

Allison van de Meene Author

Jonathan Oakhill Author

Paul Gooley Author

Related Projects (1)

Explaining the differences in affinity and of carbohydrate binding of the glycogen-sensing enzyme, AMPK

This project will provide fundamental molecular knowledge of how a complex enzyme, AMPK is controlled by the major sugar molecule, glycogen...

Grants

Awarded by Australian Research Council

Funding Acknowledgements

This work was supported by Australian Research Council Discovery Grant DP110103161 to P.R.G. and D.S., and by the National Health and Medical Research Council (J.S.O., B.E.K. and J.W.S.), supported in part by the Victorian Government's Operational Infrastructure Support Program. A.B, I.E.M, A.M.L.vdM. and M.S.D acknowledge support of the Australian Research Council Centre of Excellence in Plant Cell Walls. S.E. was the recipient of the Sir John and Lady Higgins Research Scholarship of the University of Melbourne.